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Charakterisierung und Differenzierung fluoreszierender Pseudomonaden mit Hilfe von Substratverwertungsstudien
Author(s) -
Schöpp W.,
Toaspern Cornelia,
Tauchert H.
Publication year - 1985
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.3620250307
Subject(s) - bacteria , chemistry , agar , pseudomonas , incubation , microbiology and biotechnology , agar plate , environmental chemistry , chromatography , biology , biochemistry , genetics
Abstract A rapid procedure is described for detecting and differentiating pseudomonads by their ability to degrade substrates with diverse physico‐chemical properties, including volatile and non‐volatile water‐insoluble compounds, strong organic acids and bases, surfactants, disinfectants, and other toxic substances. The bacteria are embedded in an agar medium containing mineral salts and exposed to about 5 mg amounts of six to eight different substrates placed on the surface of an agar plate. After incubation at 25°C for 24 hrs, growth zones around utilizable substrates may be used to differentiate various Pseudomonas strains and species.