Premium
Isolation, characterization, and interaction of lignin‐degrading bacteria from rumen of buffalo ( Bubalus bubalis )
Author(s) -
Wang Zhen,
Wu Wenqing,
Cui Luncheng,
Li Xiang,
Kulyar Muhammad FakhareAlam,
Xiong Haiqian,
Zhou Nian,
Yin Huaihui,
Li Jiakui,
Li Xiang
Publication year - 2021
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.202100068
Subject(s) - rumen , lignin , bubalus , bacteria , klebsiella pneumoniae , 16s ribosomal rna , biology , chemistry , food science , microbiology and biotechnology , biochemistry , botany , escherichia coli , fermentation , gene , ecology , genetics
The purpose of this study was to isolate lignin‐degrading bacteria from buffalo rumen and to explore their interactions further. Using lignin as the carbon source, three bacteria, B‐04 ( Ochrobactrum pseudintermedium ), B‐11 ( Klebsiella pneumoniae ), and B‐45 ( Bacillus sonorensis ), which have shown lignin degradation potential, were successfully isolated and identified from the rumen fluid of buffalo by colony morphology, 16S ribosomal RNA gene sequencing, and biochemical and physiological analyses. The degradation rates of lignin were determined, and the maximum values were 4.86%, 11.1%, and 7.68% for B‐04, B‐11, and B‐45, respectively. The maximum laccase activities were 0.65, 0.93, and 1.15 U/ml, while the maximum lignin peroxidase activities were 5.72, 8.29, and 18.69 U/ml, respectively. Pairwise interaction studies showed inhibitory interaction between B‐04 and B‐45, inhibitory interaction between B‐04 and B‐11, and symbiotic interaction between B‐11 and B‐45. This is the first report on the lignin degradation ability of bacteria isolated from the buffalo's rumen, which provides a new understanding for revealing the mechanism of roughage tolerance of buffalo.