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The extremophile Anoxybacillus sp. PC2 isolated from Brazilian semiarid region (Caatinga) produces a thermostable keratinase
Author(s) -
Reis Sharon V. dos,
BeysdaSilva Walter O.,
Tirloni Lucas,
Santi Lucélia,
Seixas Adriana,
Termigi Carlos,
Silva Márcia V. da,
Macedo Alexandre J.
Publication year - 2020
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.202000186
Subject(s) - keratinase , thermophile , extremophile , bioconversion , biology , bacteria , thermolysin , microbiology and biotechnology , prokaryote , groel , chemistry , food science , enzyme , biochemistry , escherichia coli , fermentation , trypsin , genetics , gene
The aim of this study was to select and identify thermophilic bacteria from Caatinga biome (Brazil) able to produce thermoactive keratinases and characterize the keratinase produced by the selected isolate. After enrichment in keratin culture media, an Anoxybacillus caldiproteolyticus PC2 was isolated. This thermotolerant isolate presents a remarkable feature producing a thermostable keratinase at 60°C. The partially purified keratinase, identified as a thermolysin‐like peptidase, was active at a pH range of 5.0–10.0 with maximal activity at a temperature range of 50–80°C. The optimal activity was observed at pH 7.0 and 50–60°C. These characteristics are potentially useful for biotechnological purposes such as processing and bioconversion of keratin.