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Efficacy of soil‐borne Enterobacter sp. for carbofuran degradation: HPLC quantitation of degradation rate
Author(s) -
Ekram Md. AkhtarE,
Sarker Indrani,
Rahi Md. Sifat,
Rahman Md. Atikur,
Saha Ananda K.,
Reza Md. Abu
Publication year - 2020
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201900570
Subject(s) - carbofuran , high performance liquid chromatography , enterobacter , ceftazidime , ciprofloxacin , bioaugmentation , biology , microbiology and biotechnology , chemistry , bioremediation , pesticide , bacteria , chromatography , antibiotics , biochemistry , ecology , genetics , escherichia coli , gene , pseudomonas aeruginosa
Excessive use of pesticides in agricultural fields is a matter of great concern for living beings as well as the environment across the world, in particular, the third world countries. Therefore, there is an urgent need to find out an effective way to degrade these hazardous chemicals from the soil in an environment‐friendly way. In the current project, a bacterial species were isolated through enrichment culture from carbofuran‐supplemented rice‐field soil and identified as a carbofuran degrader. The rate of carbofuran degradation by this bacterial species was evaluated using reverse‐phase high‐performance liquid chromatography (RP‐HPLC), which confirmed the ability to utilize as a carbon source up to 4 µg/ml of 99% technical grade carbofuran. The morphological, physiological, biochemical characteristics and phylogenetic analysis of the 16S rRNA sequence showed that this strain belongs to the genus of Enterobacter sp. (sequence accession number LC368285 in DDBJ), and the optimum growth condition for the isolated strain was 37°C at pH 7.0. Moreover, an antibiotic sensitivity test showed that it was susceptible to azithromycin, penicillin, ceftazidime, ciprofloxacin, and gentamycin, and the minimal inhibitory concentration value of gentamycin was 400 μg/ml against the bacteria. It shows beyond doubt from the RP‐HPLC quantification that the isolated bacterium has the ability to detoxify carbofuran (99% pure). Finally, the obtained results imply that the isolated strain of Enterobacter can be used as a potential and effective carbofuran degrader for bioremediation of contaminated sites through bioaugmentation.