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Analysis of genome sequence and trehalose lipid production peculiarities of the thermotolerant Gordonia strain
Author(s) -
Delegan Yanina,
Sargsyan Armen,
Hovhannisyan Nelli,
Babayan Bella,
Petrikov Kirill,
Vainstein Mikhail
Publication year - 2020
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201900439
Subject(s) - trehalose , strain (injury) , actinobacteria , biochemistry , biosynthesis , chemistry , gene , biology , whole genome sequencing , starch , microbiology and biotechnology , genome , food science , 16s ribosomal rna , anatomy
Gordoniae are one of the most promising hydrocarbon‐oxidizing actinobacteria. Here we present the genome sequence analysis of thermotolerant strain Gordonia sp. 1D isolated from oil‐refinery soil. It is capable of alkane consumption and biosurfactant production at temperatures of up to 50°C. Gordonia sp. 1D demonstrates maximum biosurfactant production when grown on hexadecane, and at 40°C it was slightly higher than at 27°C: 35 and 39 mN/m, respectively. For the first time, it was experimentally confirmed that the carbohydrate component of extracellular biosurfactants produced by strain 1D is trehalose. In addition, genes for the production of trehalose lipid biosurfactants were identified. The genetic determinants for two different pathways for trehalose synthesis were found. The strain carries genes ots A and ots B involved in de novo trehalose biosynthesis. Moreover, the genes tre Y and tre Z responsible for trehalose biosynthesis from maltooligosaccharides and starch or glycogen were identified.