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Contribution of the colicin receptor CirA to biofilm formation, antibotic resistance, and pathogenicity of Salmonella Enteritidis
Author(s) -
Zhang Zhiqiang,
Du Wannian,
Wang Miao,
Li Yonghui,
Su Shuoqing,
Wu Tonglei,
Kang Yuanhuan,
Shan Xiaofeng,
Shi Qiumei,
Zhu Guoqiang
Publication year - 2020
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201900418
Subject(s) - salmonella enteritidis , microbiology and biotechnology , biology , virulence , biofilm , salmonella , mutant , pathogen , human pathogen , bacteria , gene , genetics
Salmonella Enteritidis is an important foodborne pathogen that can infect a wide range of animal species including human beings, resulting in great losses to commercial husbandry and human health. CirA is an outer membrane receptor involved in iron uptake and colicin1A/B‐mediated competitive killing. Although iron uptake is crucial to bacterial virulence, limited literature is available about the role of CirA in infection. In the present work, we aimed to evaluate the role of CirA during S. Enteritidis infection. For this purpose, we generated a CirA‐deficient mutant of the S. Enteritidis strain C50336 and examined its biological characteristics. The results showed that cirA gene inactivation caused sharply decreased biofilm formation and apparently impaired antibiotic resistance. Furthermore, the cirA gene deletion mutant showed markedly reduced adhesion and invasion to human epithelial cell line Caco‐2 cells and decreased proliferation in mouse macrophage cell line RAW264.7 cells. Moreover, attenuated virulence was determined by a mouse model, with an LD 50 increase of approximately 1,000‐fold. These data indicated that CirA plays critical roles in the S. Enteritidis infection process.