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Sustainable use of the spent mushroom substrate of Pleurotus florida for production of lignocellulolytic enzymes
Author(s) -
Rajavat Asha S.,
Rai Sudheer,
Pandiyan Kuppusamy,
Kushwaha Prity,
Choudhary Prassan,
Kumar Murugan,
Chakdar Hillol,
Singh Arjun,
Karthikeyan Nanjappan,
Bagul Samadhan Y.,
Agnihotri Abha,
Saxena Anil K.
Publication year - 2020
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201900382
Subject(s) - xylanase , cellulase , food science , pleurotus , mushroom , chemistry , fermentation , solid state fermentation , laccase , trichoderma , botany , horticulture , enzyme , biology , biochemistry
Spent mushroom substrate (SMS), a major byproduct of the mushroom industry, is a lignocellulosic biomass, which contains approximately 57–74.3% of holocellulose fraction. This study was aimed at utilizing SMS of Pleurotus florida for recovery of lignocellulolytic enzymes and sugars and also as a substrate for production of cellulolytic enzymes using different isolates of Trichoderma and Aspergillus under solid‐state fermentation (SSF). SMS of P. florida extracts contained significant amounts of laccase (3,015.8 ± 29.5 U/g SMS) and xylanase (1,187.9 ± 12 U/g SMS) activity. Crystallinity pattern and chemical changes in SMS revealed that SMS had a lower crystallinity index (34.2%) as compared with the raw biomass (37.8%), which, in turn, helps in enhancing the accessibility of cellulolytic enzymes to holocellulose. Among the isolates, Trichoderma longibrachiatum A‐01 showed maximum activity of endoglucanase (220.4 ± 5.9 U/mg), exoglucanase (78.5 ± 3.2 U/mg) and xylanase (1,550.4 ± 11.6 U/mg) while Aspergillus aculeatus C‐08 showed maximum activity of cellobiase (113.9 ± 3.9 U/mg). Extraction with sodium citrate buffer (pH 4.8) showed maximum cellulolytic enzyme activity as compared with other solvents tested. Partial purification of endoglucanase, exoglucanase, xylanase, and cellobiase resulted in 56.3% (1,112.5 U/mg), 48.4% (212.5 U/mg), 44% (4,492.3 U/mg), and 62% (705.0 U/mg) yield with an increase by 5.2‐, 4.5‐, 4.1‐, and 5.0‐fold as compared with crude extract. The results reveal that SMS from P. florida could be a potential and cost‐effective substrate for production of cellulolytic enzymes from T. longibrachiatum A‐01 and A. aculeatus C‐08.