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Heterologous expression of the puuE from Oenococcus oeni SD‐2a in Lactobacillus plantarum WCFS1 improves ethanol tolerance
Author(s) -
Yuan Lin,
Zhao Hongyu,
Liu Longxiang,
Peng Shuai,
Li Hua,
Wang Hua
Publication year - 2019
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201900339
Subject(s) - oenococcus oeni , lactobacillus plantarum , malolactic fermentation , heterologous expression , heterologous , superoxide dismutase , microbiology and biotechnology , biochemistry , food science , lactobacillus , biology , chemistry , ethanol , fermentation , reactive oxygen species , bacteria , antioxidant , lactic acid , gene , recombinant dna , genetics
Oenococcus oeni is the main bacteria extensively used in malolactic fermentation due to its high tolerance against stress factors in wine production. Among these, ethanol is one of the main challenges to O. oeni , and its ethanol tolerance mechanism remains unclear. In this study, the puuE gene related to ethanol tolerance from O. oeni SD‐2a was heterologously expressed in Lactobacillus plantarum WCFS1. Results showed that the recombinant strain (W‐pMG36e puuE ) exhibited better growth performance and survival rate compared to the control strain (W‐pMG36e) under ethanol‐stress conditions. In addition, it was found that the activities of superoxide dismutase and the concentration of glutathione of W‐pMG36e puuE were significantly higher than those of W‐pMG36e. This resulted in the decrease of intracellular reactive oxygen species (ROS) accumulation (10.34% lower than control). Moreover, heterologous expression of puuE in WCFS1 exhibited improved activities of two ATPases in membrane, increasing the cell membrane integrity (37.67% higher than control). These results revealed the role of the puuE gene in improving ethanol tolerance in O. oeni by decreasing ROS accumulation and enhancing cell membrane integrity.