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czcD gene from Bacillus megaterium and Microbacterium liquefaciens as a potential nickel–vanadium soil pollution biomarker
Author(s) -
FierrosRomero Grisel,
GómezRamírez Marlenne,
Sharma Ashutosh,
Pless Reynaldo C.,
RojasAvelizapa Norma G.
Publication year - 2020
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201900323
Subject(s) - bacillus megaterium , microbacterium , environmental chemistry , bacteria , chemistry , microbiology and biotechnology , biology , gene expression , environmental pollution , gene , food science , biochemistry , genetics , environmental science , environmental protection , 16s ribosomal rna
Metals are among the most prevalent pollutants released into the environment. For these reasons, the use of biomarkers for environmental monitoring of individuals and populations exposed to metal pollution has gained considerable attention, offering fast and sensitive detection of chemical stress in organisms. There are different metal resistance genes in bacteria that can be used as biomarkers, including cation diffusion facilitators carrying metal ions; the prototype is the cobalt–zinc–cadmium transporter ( czcD ). The present study reports the expression changes in the czcD gene in Bacillus megaterium and Microbacterium liquefaciens under nickel and vanadium exposure by real‐time polymerase chain reaction. The nickel–vanadium‐resistant strains of B. megaterium and M. liquefaciens used in this study were isolated from mine tailings in Guanajuato, Mexico. The czcD gene showed high expression under exposure to 200 ppm of Ni and 200 ppm of V during the logarithmic growth phase of M. liquefaciens in PHGII liquid media. In contrast, no changes were observed in B. megaterium during logarithmic and stationary growth, perhaps due to the gene having differential expression during the growth phases. The expression profiles obtained for czcD show the possibility of using this gene from M. liquefaciens as a biomarker of nickel and vanadium pollution in microorganisms.