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Structural characterization of polysaccharide‐coated iron oxide nanoparticles produced by Staphylococcus warneri , isolated from a thermal spring
Author(s) -
Kianpour Sedigheh,
Ebrahiminezhad Alireza,
Deyhimi Maryam,
Negahdaripour Manica,
Raee Mohammad Javad,
Mohkam Milad,
Rezaee Hamideh,
Irajie Cambyz,
Berenjian Aydin,
Ghasemi Younes
Publication year - 2019
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201800684
Subject(s) - nanoparticle , iron oxide nanoparticles , nuclear chemistry , dynamic light scattering , fourier transform infrared spectroscopy , thermogravimetric analysis , materials science , iron oxide , chemistry , chemical engineering , nanotechnology , organic chemistry , engineering
The biocompatible‐coated iron oxide nanoparticles (IONs) have attracted a great interest because of their various applications in biological science and medicine. In most cases, the toxic effect of naked iron oxide nanoparticles is completely cleared by adding a biocompatible coating, such as polysaccharides, polyethylene glycol (PEG), or biosynthesis of biocompatible‐coated IONs using microorganisms such as bacteria. In the present study, polysaccharide‐coated iron oxide nanoparticles were produced by a strain of Staphylococcus warneri isolated from a thermal spring. For identification of the isolated bacterium, 16S rRNA gene sequencing was done. Characterization of the nanoparticles was performed for the first time, using transmission electron microscopy (TEM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), X‐ray crystallography (XRD), Fourier‐transform infrared (FTIR) spectroscopy, vibrating sample magnetometer (VSM), and 3‐(4,5‐dimethylthiazol‐2‐yl)−2,5‐diphenyltetrazolium bromide (MTT) assay. Results indicated that the spherical iron oxide nanoparticles were coated by a polysaccharide (13.6%), which provided a large negative charge of −91 mV and very low saturation magnetization of around 0.28 emu/g. The result of MTT assay on MOLT‐4 cell lines showed that the percentage of viability was between 95.6% and 68.9% in the 10–100 µM of nanoparticle concentrations with a high IC 50 value, which makes it appropriate for biomedical applications such as cancer therapy.