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Isolation, identification, and characterization of diesel‐oil‐degrading bacterial strains indigenous to Changqing oil field, China
Author(s) -
Sun Wuyang,
Ali Imran,
Liu Jiwei,
Dai Min,
Cao Wenrui,
Jiang Mingyu,
Saren Gaowa,
Yu Xinke,
Peng Changsheng,
Naz Iffat
Publication year - 2019
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201800674
Subject(s) - bioremediation , 16s ribosomal rna , diesel fuel , bacteria , bacillus thuringiensis , microbiology and biotechnology , petroleum , biology , food science , chemistry , organic chemistry , genetics
In the present study, 12 indigenous diesel‐oil‐degrading bacteria were isolated from the petroleum‐contaminated soils of the Changqing oil field (Xi’an, China). Measurement of the diesel‐oil degradation rates of these strains by the gravimetric method revealed that they ranged from 42% to 66% within 2 weeks. The highest degradation rates were observed from strains CQ8‐1 (66%), CQ8‐2 (62.6%), and CQ11 (59%), which were identified as Bacillus thuringiensis , Ochrobactrum anthropi , and Bordetella bronchialis , respectively, based on their 16S rDNA sequences. Moreover, the physiological and biochemical properties of these three strains were analyzed by Gram staining, catalase, oxidase, and Voges–Proskauer tests. Transmission electron microscopy showed that all three strains were rod shaped with flagella. Gas chromatography and mass spectrometric analyses indicated that medium‐ and long‐chain n‐alkanes in diesel oil (C11–C29) were degraded to different degrees by B. thuringiensis , O. anthropi , and B. bronchialis , and the degradation rates gradually decreased as the carbon numbers increased. Overall, the results of this study indicate strains CQ8‐1, CQ8‐2, and CQ11 might be useful for environmentally friendly and cost‐effective bioremediation of oil‐contaminated soils.