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Iron regulates hexose transporters in Schizosaccharomyces pombe
Author(s) -
Özkan Egemen,
Kartal Burcu,
Yılmazer Merve,
Palabıyık Bedia
Publication year - 2019
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201800618
Subject(s) - hexose , schizosaccharomyces pombe , transporter , biochemistry , glucose transporter , gene , chemistry , biology , saccharomyces cerevisiae , enzyme , microbiology and biotechnology , insulin
This study focuses on the effect of iron on hexose transporters which perform glucose uptake. For this aim, we investigated the role of iron in glucose utilization and expression of hexose transporters in Schizosaccharomyces pombe . We applied different iron concentrations (1, 2, 5, 10 mM) to the cells grown up to mid‐logarithmic phase. According to analysis of cell viability and morphology, we determined 2 mM and 5 mM as non‐toxic and toxic doses, respectively. Besides, glucose consumption efficiency increased (1.5‐fold) in the cells which were exposed to these iron concentrations. qRT‐PCR analysis of hexose transporter genes showed that the expression of ght2 and ght8 genes were downregulated under both non‐toxic and toxic iron conditions, but that of ght5 gene was significantly decreased only by toxic iron dose. In conclusion, it was suggested for the first time in this study that the Ght5 protein, as being high affinity hexose transporter, might play a role in sensing and signaling of iron stress.