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The different roles of hcp 1 and hcp 2 of the type VI secretion system in Escherichia coli strain CE129
Author(s) -
Ding Xueyan,
Zhang Qi,
Wang Heng,
Quan Guomei,
Zhang Dong,
Ren Wenkai,
Liao Yuexia,
Xia Pengpeng,
Zhu Guoqiang
Publication year - 2018
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201800156
Subject(s) - mutant , escherichia coli , gene , fimbria , microbiology and biotechnology , biofilm , secretion , quorum sensing , chemistry , wild type , type vi secretion system , phenotype , biology , bacteria , virulence , genetics , biochemistry
Type VI secretion system (T6SS) is a secretory system found in Gram‐negative bacteria. One of the main structures for T6SS is Hcp (hemolysin co‐regulation protein) pipeline. To investigate the role of Hcp major sub‐unit genes hcp 1 and hcp 2 , we deleted hcp 1 and hcp 2 genes for constructing the in‐frame gene deletion mutants. The properties of biofilm formation and the adhesion to chicken embryo fibroblasts cells (DF1 cells) were reduced in the hcp 2 mutant. The knockout of hcp 1 and hcp 2 genes reduced the ability of the avian pathogenic Escherichia coli (APEC) strain CE129 to infect developing chicken embryos. The expression of quorum sensing (QS)‐associated genes luxS , lsrR , and pfs were down‐regulated in the hcp 1 mutant, and the expression of type 1 fimbriae gene fimA and the adhesion‐related genes fimC and papC were decreased in the hcp 2 mutant, as well as the expression of anti‐serum survival factor genes ompA and iss were inhibited in both hcp 1 and hcp 2 mutants. These results described above from this study help to further elaborate the role of HCP in APEC.