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WxcX is involved in bacterial attachment and virulence in Xanthomonas campestri s pv. campestris
Author(s) -
Liu YuFan,
Liao ChaoTsai,
Chiang YingChuan,
Li ChihEn,
Hsiao YiMin
Publication year - 2018
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201700591
Subject(s) - xanthomonas campestris , virulence , mutant , biology , xanthomonas campestris pv. campestris , transposable element , transposon mutagenesis , mutagenesis , microbiology and biotechnology , xanthomonas , gene , escherichia coli , genetics
Xanthomonas campestris pv. campestris (Xcc) is the causative agent of black rot in crucifers. Here, one EZ‐Tn 5 transposon mutant of Xcc, altered in bacterial attachment, was isolated. Further analysis revealed that the transposon was inserted in the wxcX gene (encodes a hypothetical protein) of the transposon mutant. Sequence analysis revealed that WxcX is highly conserved in Xanthomonas , but none has been characterized. In this study, it was indicated that mutation of wxcX resulted in enhanced bacterial attachment, reduced virulence on the host cabbage, and increased sensitivity to sodium dodecyl sulfate. The affected phenotypes of the wxcX mutant could be complemented to wild‐type levels by the intact wxcX gene. Site‐directed mutagenesis revealed that E408 and E411 are critical amino acid residues for WxcX function in bacterial attachment. Taken together, our results demonstrate the roles of wxcX in attachment, virulence, and tolerance to sodium dodecyl sulfate in Xanthomonas for the first time.