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Metal‐citrate complex transport in Kineococcus radiotolerans
Author(s) -
Huta Brian P.,
Miller Nigel H.,
Robertson Eleanor L.,
Doyle Robert P.
Publication year - 2018
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201700427
Subject(s) - metal , chemistry , internalization , metal ions in aqueous solution , biochemistry , calcium , nutrient , bioinorganic chemistry , permease , biophysics , transporter , biology , gene , receptor , organic chemistry
The growth of an organism is highly dependent on the acquisition of carbon and metals, and availability of these nutrients in the environment affects its survival. Organisms can obtain both nutrients simultaneously through proteins of the CitMHS superfamily. Bioinformatic studies suggested a CitMHS gene (Accession number ABS03965.1) in Kineococcus radiotolerans . Radio flux assays following 14‐C radiolabelled citrate, either free or complexed to a variety of metal ions, in K. radiotolerans demonstrated internalization of the citrate when bound to select metal ions only, primarily in the form of calcium‐citrate. A pH response was also observed, consistent with a permease (ATP independent) mechanism as noted for other CitMHS family members, with greater uptake at pH 7 compared to pH 10. These results confirm the ability of K. radiotolerans to transport complexed citrate.