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Structural analysis and enhanced production of fusaricidin from Paenibacillus kribbensis CU01 isolated from yellow loess
Author(s) -
Ryu Jaewon,
Kim Jong M.,
Lee Chul W.,
Kim Si W.
Publication year - 2017
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201600692
Subject(s) - chromatography , chemistry , mass spectrometry , centrifugation , ion chromatography , desorption , paenibacillus polymyxa , hydrolysis , bacteria , biochemistry , biology , organic chemistry , adsorption , genetics
A bacterial strain showing strong antifungal activity was isolated from yellow loess and was identified as Paenibacillus kribbensis CU01. Insoluble mucoidal polymers were separated from M9 culture medium via low‐speed centrifugation. Most antifungal activity was associated with substances in the insoluble precipitate, which was purified by reverse phase high performance liquid chromatography. Purified fractions were analyzed using matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight mass spectrometry. Two major ion peaks with mass‐to‐charge ratio values ( m/z ) at 883.6 and 897.6 were revealed. After alkaline hydrolysis and sequence analysis, two cyclic depsipeptides were identified as, fusaricidin A and fusaricidin B. Their production was significantly increased by the addition of glucose, Fe 2+ , and Mn 2+ to M9 medium. Maximum concentrations of produced fusaricidin A and fusaricidin B at flask‐scale comprised 460 mg L −1 and 118 mg L −1 , respectively: the highest production concentrations yet reported in the literature. This demonstrates that P. kribbensis CU01 has enormous commercial potential for the mass production of fusaricidin.