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Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high‐throughput sequencing
Author(s) -
Duan Deyong,
Cheng Tianyin
Publication year - 2017
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201600557
Subject(s) - biology , nymph , wolbachia , 16s ribosomal rna , larva , zoology , microbiology and biotechnology , botany , bacteria , genetics
In this study, we analyzed the in vivo microbial community structure and diversity differences of Haemaphysalis flava ( H. flava ) in four developmental stages (egg, larva, nymph, and adult) to determine which bacterial genera could be propagated through transovarial transmission and transmitted by transstadial transmission during different developmental stages of H. flava . Paired‐end sequencing of the V3 region of the 16S ribosomal DNA (16S rDNA) of H. flava in four developmental stage samples was conducted using the Illumina MiSeq sequencing platform. The following operational taxonomic units (OTUs) of H. flava were obtained during the four developmental stages: 89 of egg, 111 of larva, 104 of nymph, and 106 of female adult tick. Sixty‐four of these OTUs had high similarity in the four developmental stages of H. flava . Eight bacterial genera had the highest abundances in all developmental stages, namely, Rickettsia , Coxiella , Pseudomonas , Ehrlichia , Escherichia , Acinetobacter , Citrobacter , and Cupriavidus . The nymph had the highest abundance of Coxiella , and the female adult tick had the highest abundance of Rickettsia . Staphylococcus and Wolbachia were detected in all developmental stages except the egg.