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Antioxidant enzymes expression in Pseudomonas aeruginosa exposed to UV‐C radiation
Author(s) -
Salma Kloula Ben Ghorbal,
Lobna Maalej,
Sana Khefacha,
Kalthoum Chourabi,
Imene Ouzari,
Abdelwaheb Chatti
Publication year - 2016
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500753
Subject(s) - superoxide dismutase , catalase , antioxidant , reactive oxygen species , biochemistry , lipid peroxidation , peroxidase , oxidative stress , chemistry , dismutase , enzyme , isozyme
It was well known that, UV‐C irradiation increase considerably the reactive oxygen species (ROS) levels in eukaryotic and prokaryotic organisms. In the enzymatic ROS‐scavenging pathways, superoxide dismutase (SOD), Catalase (CAT), and peroxidase (POX) were developed to deal with oxidative stress. In this study, we investigated the effects of UV‐C radiations on antioxidant enzymes (catalase, superoxide dismutase, and peroxidases) expression in Pseudomonas aeruginosa . Catalase, superoxide dismutase, and peroxidases activities were determined spectrophotometrically. Isozymes of superoxide dismutase were revealed by native gel activity staining method. Lipid peroxidation was determined by measuring malondialdehyde formation. Our results showed that superoxide dismutase, catalase and peroxidase activities exhibited a gradual increase during the exposure time (30 min). However, the superoxide dismutase activity was maximized at 15 min. Native gel activity staining assays showed the presence of three superoxide dismutase isozymes. The iron‐cofactored isoform activity was altered after exposure to UV‐C stress. These finding suggest that catalase and peroxidase enzymes have the same importance toward UV‐C rays at shorter and longer exposure times and this may confer additional protection to superoxide dismutase from damage caused by lipid peroxidation. Moreover, our data demonstrate the significant role of the antioxidant system in the resistance of this important human pathogen.