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Isolation and identification of biocontrol agent Streptomyces rimosus M527 against Fusarium oxysporum f. sp. cucumerinum
Author(s) -
Lu Dandan,
Ma Zheng,
Xu Xianhao,
Yu Xiaoping
Publication year - 2016
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500666
Subject(s) - fusarium oxysporum , biology , fusarium wilt , spore germination , seedling , biological pest control , botany , fusarium , streptomyces , microbiology and biotechnology , spore , horticulture , bacteria , genetics
Actinomycetes have received considerable attention as biocontrol agents against fungal plant pathogens and as plant growth promoters. In this study, a total of 320 actinomycetes were isolated from various habitats in China. Among which, 77 strains have been identified as antagonistic activities against Fusarium oxysporum f. sp . cucumerinum which usually caused fusarium wilt of cucumber. Of these, isolate actinomycete M527 not only displayed broad‐spectrum antifungal activity but also showed the strongest antagonistic activity against the spore germination of F. oxysporum f. sp . cucumerinum . In pot experiments, the results indicated that isolate M527 could promote the shoot growth and prevent the development of the disease on cucumber caused by F. oxysporum f. sp . cucumerinum . The control efficacy against seedling fusarium wilt of cucumber after M527 fermentation broth root‐irrigation was up to 72.1% as compared to control. Based on 16S rDNA sequence analysis, the isolate M527 was identified as Streptomyces rimosus .