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Expression of a chitin deacetylase gene, up‐regulated in Cryptococcus laurentii strain RY1, under nitrogen limitation
Author(s) -
Chakraborty Writachit,
Sarkar Soumyadev,
Chakravorty Somnath,
Bhattacharya Semantee,
Bhattacharya Debanjana,
Gachhui Ratan
Publication year - 2016
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500596
Subject(s) - chitin , strain (injury) , chitin synthase , lysis , biology , gene , enzyme , microbiology and biotechnology , cryptococcus , chemistry , biochemistry , chitosan , anatomy
This study reports the identification of a chitin deacetylase gene in Cryptococcus laurentii strain RY1 over‐expressing under nitrogen limitation by differential display. The up‐regulation took place in robustly growing cells rather than in starving quiescent autophagic cells. Quantitative Real Time‐PCR, enzyme activity in cell lysate and cell wall analysis corroborated the up‐regulation of chitin deacetylase under nitrogen limitation. These results suggest chitin deacetylase might play a significant role in nitrogen limiting growth of Cryptococcus laurentii strain RY1.