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Detection of ureolytic activity of bacterial strains isolated from entomopathogenic nematodes using infrared spectroscopy
Author(s) -
Lechowicz Lukasz,
Chrapek Magdalena,
Czerwonka Grzegorz,
KorzeniowskaKowal Agnieszka,
Tobiasz Anna,
Urbaniak Mariusz,
MatuskaLyzwa Joanna,
Kaca Wieslaw
Publication year - 2016
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500538
Subject(s) - infrared spectroscopy , microbiology and biotechnology , chemistry , infrared , biology , organic chemistry , physics , optics
The pathogenicity of entomopathogenic nematodes (EPNs) depends directly on the presence of bacteria in the nematode digestive tracts. Based on 16S rRNA and MALDI‐TOF analyses 20 isolated bacteria were assigned to 10 species with 10 isolates classified as Pseudomonas ssp. Six strains (30%) show ureolytic activity on Christensen medium. Spectroscopic analysis of the strains showed that the ureolytic activity is strongly correlated with the following wavenumbers: 935 cm −1 in window W4, which carries information about the bacterial cell wall construction and 1158 cm −1 in window W3 which corresponds to proteins in bacterial cell. A logistic regression model designed on the basis of the selected wavenumbers differentiates ureolytic from non‐ureolytic bacterial strains with an accuracy of 100%. Spectroscopic studies and mathematical analyses made it possible to differentiate EPN‐associated Pseudomonas sp. strains from clinical Pseudomonas aeruginosa PAO1. These results suggest, that infrared spectra of EPN‐associated Pseudomonas sp. strains may reflect its adaptation to the host.