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Enhancement of tolerance of Ganoderma lucidum to cadmium by nitric oxide
Author(s) -
Guo Shanshan,
Yao Yuan,
Zuo Lei,
Shi Wenjin,
Gao Ni,
Xu Heng
Publication year - 2016
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500451
Subject(s) - catalase , nitric oxide , mycelium , lipid peroxidation , cadmium , chemistry , tbars , antioxidant , ganoderma , toxicity , oxidative stress , proline , biochemistry , peroxidase , ganoderma lucidum , food science , biology , enzyme , botany , amino acid , organic chemistry
Nitric oxide (NO) is considered as a signaling molecule involved in regulation of diverse physiological processes and stress responses in animals and plants. However, whether NO regulates fungal, particularly edible fungi, response to heavy metal stresses, is unknown. This study investigated the effect of nitric oxide on biological responses of mycelia of Ganoderma lucidum to cadmium (Cd) toxicity. Exposure of Ganoderma lucidum to Cd (400 µM) triggered production of H 2 O 2 and O 2 − in the mycelia and further induced lipid peroxidation as well as sharply decrease of fresh biomass. However, such an effect can be reversed by exogenous supply of NO. Mycelia treated with 100 µM SNP accumulated less H 2 O 2 , O 2 – , thiobarbituric acid reactive substances (TBARS), and fresh biomass of this treatment was improved. Treatment with SNP significantly increased activities of antioxidant enzyme (peroxidase and catalase) to resist Cd stress. Meanwhile, NO‐mediated alleviation of Cd toxicity was closely related to the accumulated proline as well as reduced Cd accumulation. These results suggested that NO plays a crucial role in preventing the mycelia of Ganoderma lucidum from Cd toxicity.