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Dehydrogenase genes in the ectomycorrhizal fungus Tricholoma vaccinum : A role for Ald1 in mycorrhizal symbiosis
Author(s) -
Henke Catarina,
Jung ElkeMartina,
Voit Annekatrin,
Kothe Erika,
Krause Katrin
Publication year - 2016
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500381
Subject(s) - ectomycorrhiza , symbiosis , biology , aldehyde dehydrogenase , hypha , mycorrhiza , fungus , botany , indole 3 acetic acid , auxin , biochemistry , gene , bacteria , genetics
Ectomycorrhizal symbiosis is important for forest ecosystem functioning with tree‐fungal cooperation increasing performance and countering stress conditions. Aldehyde dehydrogenases (ALDHs) are key enzymes for detoxification and thus may play a role in stress response of the symbiotic association. With this focus, eight dehydrogenases, Ald1 through Ald7 and TyrA, of the ectomycorrhizal basidiomycete Tricholoma vaccinum were characterized and phylogenetically investigated. Functional analysis was performed through differential expression analysis by feeding different, environmentally important substances. A strong effect of indole‐3‐acetic acid (IAA) was identified, linking mycorrhiza formation and auxin signaling between the symbiosis partners. We investigated ald1 overexpressing strains for performance in mycorrhiza with the host tree spruce ( Picea abies ) and observed an increased width of the apoplast, accommodating the Hartig’ net hyphae of the T. vaccinum over‐expressing transformants. The results support a role for Ald1 in ectomycorrhiza formation and underline functional differentiation within fungal aldehyde dehydrogenases in the family 1 of ALDHs.