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Biodegradation of paraffin wax by crude Aspergillus enzyme preparations for potential use in removing paraffin deposits
Author(s) -
Zhang Junhui,
Xue Quanhong,
Gao Hui,
Wang Ping
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500290
Subject(s) - wax , paraffin wax , biodegradation , chemistry , liquid paraffin , chromatography , propionate , organic chemistry , materials science , composite material
Paraffin deposition problems have plagued the oil industry. Whist mechanical and chemical methods are problematic, microbiological method of paraffin removal is considered an alternative. However, studies have mainly investigated the use of bacteria, with little attention to the potential of fungi. The performance of six Aspergillus isolates to degrade paraffin wax was evaluated under laboratory conditions using solid enzyme preparations. The results showed that all the six enzyme preparations efficiently improved the solubility of paraffin wax in n‐ hexane and degraded n‐ alkanes in paraffin wax. The degradation process was accompanied by dynamic production of gases (CO 2 and H 2 ) and organic acids (oxalate and propionate). The shape of wax crystals markedly changed after enzymatic degradation, with a rough surface and a loose structure. This study indicates that extracellular enzymes from Aspergillus spp. can efficiently degrade paraffin wax. These enzyme preparations have the potential for use in oil wells with paraffin deposition problems.