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Thermus anatoliensis sp. nov., a thermophilic bacterium from geothermal waters of Buharkent, Turkey
Author(s) -
Kacagan Murat,
Inan Kadriye,
Canakci Sabriye,
Guler Halil Ibrahim,
Belduz Ali Osman
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500282
Subject(s) - thermus , 16s ribosomal rna , thermophile , biology , microbiology and biotechnology , bacteria , strain (injury) , glycolipid , biochemistry , genetics , anatomy
A Gram‐stain‐negative, lack of motility, catalase‐ and oxidase‐ positive bacterium (strain MT1 T ) was isolated from Buharkent hot spring in Aydin, Turkey. Its taxonomy was investigated using a polyphasic approach. The strain was able to grow at 45–80 °C, pH 5.5–10.5 and with a NaCI tolerance up to 2.0% (w/v). Strain MT1 T was able to utilize d ‐mannitol and l ‐arabinose, not able to utilize d ‐cellobiose as sole carbon source. 16S rRNA gene sequence analysis revealed that the strain belonged to the genus Thermus; strain MT1 T detected low‐level similarities of 16S rRNA gene sequences (below 97%) compared with all other species in this genus. The predominant fatty acids of strain MT1 T were iso‐C 15:0 (43.0%) and iso‐C 17:0 (27.4%). Polar lipid analysis revealed a major phospholipid, one major glycolipid, one major aminophospholipid, two minor aminolipids, one minor phospholipid, and several minor glycolipids. The major isoprenoid quinone was MK‐8. The DNA G+C content of MT1 T was 69.6 mol%. On the basis of a taxonomic study using a polyphasic approach, strain MT1 T is considered to represent a novel species of the genus Thermus , for which the name Thermus anatoliensis sp. nov. is proposed. The type strain is MT1 T (=NCCB 100425 T =LMG 26880 T ).