Premium
Aeromonas rivipollensis sp. nov., a novel species isolated from aquatic samples
Author(s) -
Marti Elisabet,
Balcázar José Luis
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500264
Subject(s) - biology , aeromonas hydrophila , aeromonas , phylogenetic tree , 16s ribosomal rna , microbiology and biotechnology , bacteria , sequence analysis , gene , phylogenetics , vibrionaceae , ribosomal rna , genetics
Two gram‐negative, facultatively anaerobic, motile and rod‐shaped bacteria, strains P2G1 T and P1A11, were isolated from the Ter River in Ripoll, Spain. Phylogenetic analysis based on 16S rRNA gene sequences showed that both strains are closely related to each other and that their closest relatives were Aeromonas media ATCC 33907 T (99.4%) and Aeromonas hydrophila ATCC 7966 T (99.3%). Multilocus sequence analysis (MLSA) based on the partial sequences of gyrA , gyrB , rpoD , recA , and dnaJ genes suggested that these two strains represent a novel species that clustered with A. media ATCC 33907 T . This was further supported by DNA–DNA hybridization analysis between P2G1 T and A. media LMG 9073 T . Phenotypic features also allowed their differentiation from closely related species. These two strains should, therefore, be considered to represent a novel species within the genus Aeromonas , for which the name Aeromonas rivipollensis sp. nov. is proposed.