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Effect of heavy metals on acdS gene expression in Herbaspirillium sp. GW103 isolated from rhizosphere soil
Author(s) -
Loganathan Praburaman,
Myung Hyun,
Muthusamy Govarthanan,
Lee KuiJae,
Seralathan KamalaKannan,
Oh ByungTaek
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201500008
Subject(s) - incubation , deamination , rhizosphere , gene expression , gene , strain (injury) , enzyme , chemistry , microbiology and biotechnology , biology , biochemistry , bacteria , genetics , anatomy
This study aimed to understand the influence of heavy metals on 1‐aminocyclopropane‐1‐carboxylate deaminase activity (ACCD) and acdS gene expression in Herbaspirillium sp. GW103. The GW103 strain ACCD activity decreased in cells grown in a medium supplemented with Pb and As, whereas cells grown in medium supplemented with Cu showed increase in enzyme activity. The GW103 strain produced 262.2 ± 6.17 μmol of α‐ketobutyrate per milligram of protein per hour during ACC deamination at 25 °C after 24 h incubation. Using a PCR approach, an acdS coding‐gene of 1.06 kbp was amplified in isolate GW103, showing 92% identity with Herbaspirillum seropedicae SmR1 acdS gene. Real time quantitative polymerase chain reaction results indicate that the acdS expression rate was increased (7.1‐fold) in the presence of Cu, whereas it decreased (0.2‐ and 0.1‐fold) in the presence of As and Pb.