Premium
Bacillus radicibacter sp. nov., a new bacterium isolated from root nodule of Oxytropis ochrocephala Bunge
Author(s) -
Wei Xiu Li,
Lin Yan Bing,
Xu Lin,
Han Meng Sha,
Dong Dan Hong,
Chen Wei Min,
Wang Li,
Wei Ge Hong
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400940
Subject(s) - 16s ribosomal rna , biology , phosphatidylglycerol , bacillus (shape) , strain (injury) , bacteria , microbiology and biotechnology , phylogenetic tree , genus , ribosomal rna , botany , bacillaceae , terpenoid , bacillus subtilis , phospholipid , gene , biochemistry , phosphatidylcholine , genetics , anatomy , membrane
A Gram‐positive, facultative anaerobic, rod‐shaped, and endospore‐forming strain, designated 53‐2 T was isolated from the root nodule of Oxytropis ochrocephala Bunge growing on Qilian mountain, China. The strain can grow at pH 7.0–8.0, 10–50 °C and tolerate up to 11% NaCl. Optimal growth occurred at pH 7.2 and 37 °C. The result of BLASTn search based on 16S rRNA gene sequence revealed that strain 53‐2 T , being closest related to Bacillus acidicola 105‐2 T , possessed remote similarity (less than 95.64%) to the species within genus Bacillus . The DNA G + C content was 37.8%. Chemotaxonomic data (major quinone is MK‐7; major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, unknown phospholipid, and aminoglycophospholipid; fatty acids are anteiso‐C 15: 0 , iso‐C 15:0 and anteiso‐C 17: 0 ) supported the affiliation of the isolate to the genus Bacillus . On the basis of physiological, phylogenetic, and biochemical properties, strain 53‐2 T represents a novel species within genus Bacillus , for which the name Bacillus radicibacter is proposed. The type strain is 53‐2 T (=DSM27302 T =ACCC06115 T =CCNWQLS5 T ).