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Phylogeny and evolutionary genetics of Frankia strains based on 16S rRNA and nif D–K gene sequences
Author(s) -
Mishra Arun Kumar,
Singh Pawan Kumar,
Singh Prashant,
Singh Anumeha,
Singh Satya Shila,
Srivastava Amrita,
Srivastava Alok Kumar,
Sarma Hridip Kumar
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400914
Subject(s) - biology , frankia , phylogenetics , 16s ribosomal rna , ribosomal rna , phylogenetic tree , evolutionary biology , genetics , genetic diversity , gene , bacteria , nitrogen fixation , root nodule , population , demography , sociology
16S rRNA and nif D– nif K sequences were used to study the molecular phylogeny and evolutionary genetics of Frankia strains isolated from Hippöphae salicifolia D. Don growing at different altitudes (ecologically classified as riverside and hillside isolates) of the Eastern Himalayan region of North Sikkim, India. Genetic information for the small subunit rRNA (16S rRNA) revealed that the riverside Frankia isolates markedly differed from the hillside isolates suggesting that the riverside isolates are genetically compact. Further, for enhanced resolutions, the partial sequence of nif D (3′ end), nif K (5′ end) and nif D–K IGS region have been investigated. The sequences obtained, failed to separate riverside isolates and hillside isolates, thus suggesting a possible role of genetic transfer events either from hillside to riverside or vice versa. The evolutionary genetic analyses using evogenomic extrapolations of gene sequence data obtained from 16S rRNA and nif D–K provided differing equations with the pace of evolution being more appropriately, intermediate. Values of recombination frequency ( R ), nucleotide diversity per site ( Pi ), and DNA divergence estimates supported the existence of an intermixed zone where spatial isolations occurred in sync with the temporal estimates. J. Basic Microbiol. 2015, 54 , 1–9