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Phenotypic and genetic characterization of rhizobia isolated from Hedysarum flexuosum in Northwest region of Morocco
Author(s) -
Ezzakkioui Fatima,
El Mourabit Nourdin,
Chahboune Rajaa,
CastellanoHinojosa Antonio,
Bedmar Eulogio J.,
Barrijal Said
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400790
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , root nodule , rhizobia , ribosomal rna , gammaproteobacteria , phylogenetic tree , alphaproteobacteria , genetics , botany , gene , symbiosis , bacteria
Seventy bacterial strains were isolated from root nodules of the legume Hedysarum flexuosum grown wild in soils from Northwest Morocco. Repetitive extragenic palindromic (REP)‐polymerase chain reaction (PCR) clustered the strains into 30 REP‐PCR groups. The nearly complete sequence of the 16S rRNA gene from a representative strain of each REP‐PCR pattern showed that 17 strains were closely related to members of the genus Rhizobium of the family Rhizobiaceae of the Alphaproteobacteria. Pairwise alignments between globally aligned sequences of the 16S rRNA gene indicated that the strains from H. flexuosum had 99.75–100% identity with Rhizobium sullae type strain IS123 T . The phenotypic characteristics analyzed allowed description of a wide physiological diversity among the isolates, where the carbohydrate assimilation test was the most discriminating. Analysis of the 16S rRNA gene of a representative strains from the remaining 13 REP‐PCR groups showed they belong to a wide variety of phylogenetic groups being closely related to species of genera Stenotrophomonas , Serratia , Massilia , Acinetobacter , Achromobacter , and Pseudomonas from the Beta‐ and Gammaproteobacteria. The R. sullae strains identified in this study produced effective symbiosis with their original host plant. None of the other bacterial strains could form nodules on H. flexuosum .