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Proteomic analysis of Streptomyces sp. ssr‐198 grown on paddy straw
Author(s) -
Singh Surender,
Tiwari Rameshwar,
Renuse Santosh,
Pranaw Kumar,
Nain Lata
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400639
Subject(s) - cellulase , proteases , biochemistry , glycoside hydrolase , streptomyces , enzyme , polysaccharide , orbitrap , hydrolysis , proteome , chemistry , tandem mass spectrometry , bacteria , biology , enzymatic hydrolysis , microbiology and biotechnology , mass spectrometry , chromatography , genetics
The filamentous bacteria Streptomyces spp. produces diverse extracellular enzymes and other secondary metabolites. Proteomic analysis of the secretome of holocellulolytic Streptomyces sp. ssr‐198 was done by tandem mass spectrometry using an Orbitrap Velos hybrid mass spectrometer. A wide range of hydrolytic enzymes, including glycoside hydrolases (17), proteases (17), polysaccharide lyases (3), esterases (2), and hypothetical proteins (14) were detected in the secretome analyzed. Overall, the secretome composition constituted of 12.50% cellulases, 17.50% hemicellulases, 21.25% proteases, 17.50% hypothetical proteins, and 31.25% other proteins. Comprehensive analysis of secretome will be useful in gaining better understanding of the unique role of hydrolytic enzymes in lignocellulose hydrolysis and helps in determining the industrial applications of these potent enzymes.