Premium
Modulation of Caenorhabditis elegans immune response and modification of Shigella endotoxin upon interaction
Author(s) -
Kesika Periyanaina,
Prasanth Mani Iyer,
Balamurugan Krishnaswamy
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400511
Subject(s) - shigella , caenorhabditis elegans , innate immune system , biology , microbiology and biotechnology , immune system , lipopolysaccharide , pathogen , bacteria , gene , immunology , genetics , salmonella
To analyze the pathogenesis at both physiological and molecular level using the model organism, Caenorhabditis elegans at different developmental stages in response to Shigella spp. and its pathogen associated molecular patterns such as lipopolysaccharide. The solid plate and liquid culture‐based infection assays revealed that Shigella spp. infects C. elegans and had an impact on the brood size and pharyngeal pumping rate. LPS of Shigella spp. was toxic to C. elegans . qPCR analysis revealed that host innate immune genes have been modulated upon Shigella spp. infections and its LPS challenges. Non‐destructive analysis was performed to kinetically assess the alterations in LPS during interaction of Shigella spp. with C. elegans . The modulation of innate immune genes attributed the surrendering of host immune system to Shigella spp. by favoring the infection. LPS appeared to have a major role in Shigella ‐mediated pathogenesis and Shigella employs a tactic behavior of modifying its LPS content to escape from the recognition of host immune system.