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Novel co‐enrichment method for isolation of magnetotactic bacteria
Author(s) -
Sorty Ajay M.,
Shaikh Nasir R.
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400457
Subject(s) - magnetotactic bacteria , magnetosome , fastidious organism , isolation (microbiology) , bacteria , environmental chemistry , population , chemistry , in situ , soil water , biology , microbiology and biotechnology , ecology , paleontology , demography , organic chemistry , sociology
A novel co‐enrichment technique was designed for enrichment of magnetotactic bacteria from soil, water, and sediments. Delayed addition of iron uptake inducer and the iron source proved amenable to induce magnetosome synthesis by MTB followed by their separation from consortium using magnetic flux. We successfully enriched and isolated both North seeking as well as South seeking magnetotactic bacteria from Lonar Lake (Buldhana), Moti Lake (Jalna), Ghanewadi Lake (Jalna), Ganesh Lake (Miraj), Rankala Lake (Kolhapur), and industrial metal‐contaminated glaying soils (Jalna) and a soil (Karad), (MS, India) exposed to high‐voltage electric current. The hanging drop preparations and growth under magnetic stress on low‐agar media allowed conformation of magnetotactic behavior of the isolates. Both Gram positive and Gram negative MTB were isolated with diverse morphologies. South seeking population was more predominant. The soil inhabitants showed little dwelling property which was more prominent in case of aquatic inhabitants. The use of in situ pH and salt concentrations during enrichment and isolation found suited. The simultaneous growth of whole consortium in the system ensured the in situ simulation of microenvironment needful for proper growth of fastidious MTB.