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In vitro Scleroderma laeve and Eucalyptus grandis mycorrhization and analysis of atp 6, 17S rDNA, and ras gene expression during ectomycorrhizal formation
Author(s) -
de Freitas Pereira Maíra,
Betancourth Blanca Mercedes Leguízamo,
Teixeira Janaina Aparecida,
Zubieta Mariane Paludetti,
de Queiroz Marisa Vieira,
Kasuya Maria Catarina Megumi,
Costa Mauricio Dutra,
de Araújo Elza Fernandes
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400253
Subject(s) - biology , gene , gene expression , microbiology and biotechnology , ectomycorrhizae , regulation of gene expression , botany , symbiosis , genetics , mycorrhiza , bacteria
The interaction between fungi and plants that form ectomycorrhizae (ECM) promotes alterations in the gene expression profiles of both organisms. Fungal genes expression related to metabolism were evaluated at the pre‐symbiotic stage and during the ECM development between Scleroderma laeve and Eucalyptus grandis . Partial sequences of ATP synthase ( atp 6), translation elongation factor ( ef1 α), the RAS protein ( ras ), and the 17S rDNA genes were isolated. The expression of the atp 6 and 17S rDNA genes during the pre‐symbiotic stage showed an approximately threefold increase compared to the control. During ECM development, the expression of the 17S rDNA gene showed a 4.4‐fold increase after 3 days of contact, while the expression of the atp 6 gene increased 7.23‐fold by the 15th day, suggesting that protein synthesis and respiratory chain activities are increased during the formation of the mantle and the Hartig net. The ras gene transcripts were only detected by RT‐PCR 30 days after fungus–plant contact, suggesting that RAS‐mediated signal transduction pathways are functional during the establishment of symbiosis. The present study demonstrates that alterations in gene expression occur in response to stimuli released by the plant during ECM association and increases the understanding of the association between S. laeve and E. grandis .