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Overproduction of geranylgeraniol in Coprinopsis cinerea by the expression of geranylgeranyl diphosphate synthase gene
Author(s) -
You LinFeng,
Guo LiQiong,
Lin JunFang,
Ren Tao,
Wang JianRong
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400152
Subject(s) - geranylgeraniol , squalene , ergosterol , biochemistry , biosynthesis , biology , atp synthase , overproduction , metabolic engineering , terpenoid , chemistry , gene
( E , E , E )‐Geranylgeraniol (GGOH) is a valuable ingredient of many perfumes and a valuable precursor for synthesizing pharmaceuticals. In an attempt to increase the GGOH concentration in Coprinopsis cinerea , we demonstrated that the expression of geranylgeranyl diphosphate synthase ( ggpps ) gene isolated from Taxus x media could promote GGOH production. Furthermore, the concentrations of squalene and ergosterol were measured in the engineered strains. Expectedly, significant decreases of squalene and ergosterol levels were observed in those strains transformed with ggpps gene. This could be explained by the partial redirection of metabolic flux from squalene to GGOH, whose biosynthesis competes for the same precursor with squalene. This work suggested that the expression of ggpps in higher fungi was an effective method for bio‐production of GGOH.