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Physiological and biochemical characterization of Azospirillum brasilense strains commonly used as plant growth‐promoting rhizobacteria
Author(s) -
Di Salvo Luciana P.,
Silva Esdras,
Teixeira Kátia R.S.,
Cote Rosalba Esquivel,
Pereyra M. Alejandra,
García de Salamone Inés E.
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400135
Subject(s) - rhizobacteria , azospirillum brasilense , microbial inoculant , isolation (microbiology) , biology , bacteria , inoculation , microbiology and biotechnology , botany , horticulture , rhizosphere , genetics
Azospirillum is a plant growth‐promoting rhizobacteria (PGPR) genus vastly studied and utilized as agriculture inoculants. Isolation of new strains under different environmental conditions allows the access to the genetic diversity and improves the success of inoculation procedures. Historically, the isolation of this genus has been performed by the use of some traditional culture media. In this work we characterized the physiology and biochemistry of five different A. brasilense strains, commonly used as cereal inoculants. The aim of this work is to contribute to pose into revision some concepts concerning the most used protocols to isolate and characterize this bacterium. We characterized their growth in different traditional and non‐traditional culture media, evaluated some PGPR mechanisms and characterized their profiles of fatty acid methyl esters and carbon‐source utilization. This work shows, for the first time, differences in both profiles, and ACC deaminase activity of A. brasilense strains. Also, we show unexpected results obtained in some of the evaluated culture media. Results obtained here and an exhaustive knowledge revision revealed that it is not appropriate to conclude about bacterial species without analyzing several strains. Also, it is necessary to continue developing studies and laboratory techniques to improve the isolation and characterization protocols.