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Isolation and characteristics of protocatechuic acid from Paenibacillus elgii HOA73 against Botrytis cinerea on strawberry fruits
Author(s) -
Nguyen Xuan Hoa,
Naing Kyaw Wai,
Lee Young Seong,
Moon Jae Hak,
Lee Jeong Hyun,
Kim Kil Yong
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201400041
Subject(s) - botrytis cinerea , rhizoctonia solani , mycelium , biology , fungicide , protocatechuic acid , conidium , phytophthora capsici , spore germination , alternaria solani , vinclozolin , botrytis , horticulture , botany , germination , phytophthora , biochemistry , antioxidant
This study was undertaken to describe purification, identification, and characteristics of protocatechuic acid (PCA) isolated for the first time from Paenibacillus elgii HOA73 against Botrytis cinerea (the cause of gray mold disease on strawberry fruit). PCA was purified by different chromatographic techniques and identified as PCA (3,4‐dihydroxybenzoic acid) by nuclear magnetic resonance and liquid chromatography–mass spectrometry analyses. PCA displayed potent antifungal activity against B. cinerea and Rhizoctonia solani . However, the antifungal activities were not sufficient to inhibit mycelial growth of Phytophthora capsici and Fusarium oxysporum . The minimum inhibitory concentration of PCA to inhibit any visible mycelial growth of both B. cinerea and R. solani was 64 µg ml −1 . Most B. cinerea conidia displayed altered shape and absence of germination, or were degraded after treatment with 50 and 100 µg ml −1 PCA, respectively. Moreover, gray mold formation on strawberry fruit was almost or completely inhibited by these PCA concentrations 7 days following infection with B. cinerea conidia, respectively. PCA may be a promising alternative to chemical fungicides as a potential biofungicide to prevent growth of B. cinerea in strawberry fruit disease management.