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Thc6 protein, isolated from Trichoderma harzianum , can induce maize defense response against Curvularia lunata
Author(s) -
Fan Lili,
Fu Kehe,
Yu Chuanjin,
Li Yingying,
Li Yaqian,
Chen Jie
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201300814
Subject(s) - jasmonic acid , biology , mutant , methyl jasmonate , jasmonate , plant defense against herbivory , complementation , agrobacterium tumefaciens , inbred strain , transformation (genetics) , gene , trichoderma harzianum , microbiology and biotechnology , wild type , botany , genetics , arabidopsis , biological pest control
Mutant T66 was isolated from 450 mutants (constructed with Agrobacterium tumefaciens ‐mediated transformation method) of Trichoderma harzianum . Maize seeds coated with T66 were more susceptible to Curvularia lunata when compared with those coated with wild‐type (WT) strain. The disease index of maize treated with T66 and WT were 62.5 and 42.1%, respectively. Further research showed T‐DNA has inserted into the ORF of one gene, which resulted in the functional difference between WT and T66. The gene was cloned and named Thc 6, which encodes a novel 327 amino acid protein. To investigate its function, we obtained knockout, complementation, and overexpression mutants of Thc 6. Challenge inoculation studies suggested that the Thc 6 overexpression mutant can reduce the disease index of maize inbred line Huangzao 4 against the leaf spot pathogen ( C. lunata ). Meanwhile, The Thc 6 mutants were found to affect the resistance of maize inbred line Huangzao 4 against C. lunata by enhancing the activation of jasmonate‐responsive genes expression. Liquid chromatography–mass spectrometry (LC–MS) data further confirmed that the concentration of jasmonate in the induced maize exhibits a parallel change tendency with the expression level of defense‐related genes. Hence, the Thc 6 gene could be participated in the induced resistance of maize inbred line Huangzao 4 against C. lunata infection through a jasmonic acid‐dependent pathway.