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Effect of heavy metals on growth response and antioxidant defense protection in Bacillus cereus
Author(s) -
Behera Madhumita,
Dandapat Jagneshwar,
Rath Chandi Charan
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201300805
Subject(s) - catalase , chemistry , glutathione , antioxidant , oxidative stress , bacillus cereus , superoxide dismutase , reactive oxygen species , biochemistry , hydrogen peroxide , bacteria , intracellular , context (archaeology) , toxicity , bacterial growth , food science , enzyme , biology , organic chemistry , paleontology , genetics
Bacterial cells in aerobic environment generate reactive oxygen species which may lead to oxidative stress, induced by a wide range of environmental factors including heavy metals. In the present context an attempt has been made to determine the toxic impact of cadmium and copper on growth performance, oxidative stress, and relative level of antioxidant protection in Bacillus cereus . Outcome of this study suggests that both the metal ions depleted the growth rate in this organism with respect to time and concentration of the metal ions. CdCl 2 exposure induced extracellular glutathione (GSH) production, whereas, its level was declined in response to CuSO 4. Superoxide dismutase (SOD) activity and hydrogen peroxide (H 2 O 2 ) content was elevated under CdCl 2 stress but the activity of catalase (CAT) was inhibited. In contrast, incubation of bacteria with CuSO 4 exhibited decreased SOD activity with concomitant rise in CAT activity and H 2 O 2 content. We also observed elevation of intracellular GSH level in this bacteria following supplementation of N ‐acetyl cysteine (NAC) in the medium. Overall findings of this study indicated differential toxicity of CdCl 2 and CuSO 4 in inducing oxidative stress, depleting growth rate and the possible involvement of GSH and CAT in adaptive antioxidant response.