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Removal of the insecticide diazinon from liquid media by free and immobilized Streptomyces sp. isolated from agricultural soil
Author(s) -
Briceño Gabriela,
Fuentes María S.,
Rubilar Olga,
Jorquera Milko,
Tortella Gonzalo,
Palma Graciela,
Amoroso María J.,
Diez Maria Cristina
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201300576
Subject(s) - diazinon , actinobacteria , streptomyces , pesticide , chemistry , food science , microbiology and biotechnology , chromatography , biology , bacteria , biochemistry , agronomy , genetics , 16s ribosomal rna , gene
From an agricultural soil that had received continuous applications of organophosphorus pesticides, 30 actinobacteria strains were isolated. Two strains, identified as Streptomyces sp. AC1‐6 and Streptomyces sp. ISP4, were selected because of their tolerance to diazinon and based on the relationship between diazinon removal and microbial growth. In liquid medium with diazinon at concentrations of 25 and 50 mg L −1 , both strains were able to remove approximately 40–50% and 70–90% of the initial diazinon after 24 and 96 h of incubation, respectively. This diazinon removal was accompanied by microbial growth of the strains, an initial pH decrease, and glucose consumption in the liquid medium. Evaluation of the diazinon removal achieved by the free actinobacteria and Streptomyces sp. AC1‐6 immobilized on alginate beads revealed that the immobilized cells exhibited a 60% higher diazinon removal compared with the free cells. The reusability of the encapsulated biomass was confirmed, and a diazinon removal rate of more than 50% was obtained after the second batch. This work constitutes one of the few reports that describe Streptomyces strains as diazinon degraders. Given the high diazinon removal found, the streptomycetes exhibit suitable potential as diazinon‐degrading actinobacteria for elimination of diazinon from liquid residues.