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Isolation and characterization of a novel native Bacillus thuringiensis strain BRC‐HZM2 capable of degrading chlorpyrifos
Author(s) -
Wu Songqing,
Peng Yan,
Huang Zhangmin,
Huang Zhipeng,
Xu Lei,
Ivan Gelbič,
Guan Xiong,
Zhang Lingling,
Zou Shuangquan
Publication year - 2015
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201300501
Subject(s) - bacillus thuringiensis , chlorpyrifos , microbiology and biotechnology , strain (injury) , biology , 16s ribosomal rna , bioremediation , plasmid , bacteria , pesticide , food science , gene , biochemistry , genetics , anatomy , agronomy
Studies were carried out to isolate chlorpyrifos degrading Bacillus thuringiensis (Bt) strains from chlorpyrifos‐contaminated samples. Six Bt strains (isolation rate 2.7%) were isolated by modified sodium acetate antibiotic heat treatment, and one novel strain (BRC‐HZM2) was selected for further analysis. Phenotype and phylogeny analysis of this strain was conducted on the basis of biochemical reactions, antibiotic sensitivity, 16s rRNA genes, plasmid profile, insecticidal crystal protein profiles, and PCR–RFLP for cry and cyt genes. The degradation rate of chlorpyrifos in liquid culture was estimated during 48 h of incubation for the isolate BRC‐HZM2. More than 50% of the initial chlorpyrifos concentration degraded within 12 h, 88.9% after 48 h. These results highlight the potential of the Bt strain for biological control and the bioremediation of environments contaminated with chlorpyrifos.