Premium
Increase of the phytase production by Aspergillus japonicus and its biocatalyst potential on chicken feed treatment
Author(s) -
Maller Alexandre,
Vici Ana Claudia,
Facchini Fernanda Del Antonio,
da Silva Tony Marcio,
Kamimura Eliana Setsuko,
Rodrigues Maria Isabel,
Jorge João Atílio,
Terenzi Hector Francisco,
de Lourdes Teixeira de Moraes Polizeli Maria
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201300315
Subject(s) - phytase , broiler , food science , yeast extract , phytic acid , central composite design , feed additive , chemistry , biology , response surface methodology , enzyme , biochemistry , fermentation , chromatography
Phytase hydrolyzes phytic acid from the plant components of animal feed, releasing inorganic phosphorus. The phytase production by Aspergillus japonicus was optimized using Plackett–Burman designs (PBD), composite central rotational designs (CCRD), and response surface methodology from standard Czapek medium. The enzyme was applied in broiler chicken and laying hen foods. Analysis from PBD showed that KH 2 PO 2 , MgSO 4 · 7H 2 O, and yeast extract had significant influences on phytase secretion ( p < 0.05). The best results from the CCRD experiments were obtained using ( A ) 0.040% KH 2 PO 4 , ( B ) 0.050% MgSO 4 · 7H 2 O, and ( C ) 0.040% yeast extract, enhancing in 49–53 U mg −1 protein. The determination coefficient ( R 2 ) was 0.92 and F calc was 7.48 times greater than F listed . Thus, the reduced coded model: Y ( U m g − 1 ) = 50.29 + 4.30 A − 3.35 ( A ) 2 − 4.80 ( B ) 2 + 5.62 C − 4.26 ( C ) 2was considered predictive and statistically significant ( p < 0.05). The optimized culture medium increased the phytase yield in 250%. A. japonicus phytase released high levels of Pi from broiler chicken and laying hen food. A. japonicus is an excellent phytase producer in a culture medium using inexpensive components and agricultural wastes. Therefore, these results provide sound arguments for the formulation of a low cost culture medium for phytase production.