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Production of AI ‐2 is mediated by the S ‐ribosylhomocystein lyase gene lux S in Bacteroides fragilis and Bacteroides vulgatus
Author(s) -
Peixoto Rafael José Marques,
Miranda Karla Rodrigues,
Ferreira Eliane Oliveira,
de Paula Geraldo Renato,
Rocha Edson Ribeiro,
Lobo Leandro Araujo,
Domingues Regina Maria Cavalcanti Pilotto
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201300311
Subject(s) - bacteroides fragilis , bacteroides , quorum sensing , microbiology and biotechnology , biology , escherichia coli , autoinducer , gene , bacteroidaceae , bacteria , biochemistry , genetics , virulence , antibiotics
Quorum sensing is a cell–cell signaling mechanism based on cell density and that involves the production of hormone‐like molecules called autoinducers (AI). One of the most studied AIs has been termed AI‐2, and its biosynthesis requires the enzyme encoded by luxS . We have previously described for the first time that Bacteroides species can produce molecules with AI‐2 activity. In this study, we focus on the detection of luxS and its activity as the AI‐2 synthase in Bacteroides species. The strains Bacteroides fragilis B3b and Bacteroides vulgatus ATCC 8482 were selected based on a positive phenotype for AI‐2 production and the presence of a putative luxS in the genome, respectively. In order to identify the luxS gene, cloning and heterologous expression strategies were utilized. We demonstrate that both strains contain functional luxS orthologs that can complement AI‐2 production in Escherichia coli .