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An ultrasonic method for separation of epiphytic microbes from freshwater submerged macrophytes
Author(s) -
Cai Xianlei,
Gao Guang,
Yang Jing,
Tang Xiangming,
Dai Jiangyu,
Chen Dan,
Song Yuzhi
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201300041
Subject(s) - macrophyte , epiphyte , aquatic plant , ultrasonic sensor , environmental science , aquatic ecosystem , ecology , biology , physics , acoustics
Epiphytic microbes are common inhabitants of freshwater submerged macrophytes, which play an important role in aquatic ecosystems. An important precondition for studying the epiphytic microbes is having an effective method of separating the attached microbes from the host macrophytes. We developed an ultrasound‐based method for separating epiphytic microbes from freshwater submerged macrophytes, optimized the conditions of ultrasonic separation with an orthogonal experimental design, and compared the optimized ultrasonic method with manual separation. This method can be particularly useful for freshwater submerged macrophytes having a complex morphology.