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Antifungal attributes of siderophore produced by the Pseudomonas aeruginosa JAS‐25
Author(s) -
Sulochana Mudgulkar Bhimsen,
Jayachandra Sharanappa Yaradoddi,
Kumar Subhash Katti Anil,
Dayanand Agsar
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200770
Subject(s) - siderophore , fusarium oxysporum , aspergillus niger , genbank , microbiology and biotechnology , pseudomonas aeruginosa , spore germination , biology , 16s ribosomal rna , fusarium , sequence homology , pseudomonas , antifungal , biological pest control , sephadex , spore , food science , botany , bacteria , biochemistry , gene , enzyme , peptide sequence , genetics
Siderophores produced by Pseudomonas sp. play a vital role in controlling several plant diseases. Public concern about chemical pesticides has fostered an interest to seek alternative control strategies. Production of siderophore by Pseudomonas aeruginosa JAS‐25 in King B medium was estimated at 400 nm and was partially purified by gel filtration chromatography using Sephadex G 25. Antifungal activity and inhibition of spore germination was studied against the phytopathogens like Fusarium oxysporum f . sp. ciceri , Fusarium udum , and Aspergillus niger by well plate assay and microphotographic method. Zone of inhibition of 3.0 cm was observed for F. udum and A. niger , proving it as potential biocontrol agent against phytopathogens. Partial 16S rRNA sequence analysis showed that the isolate JAS‐25 exhibited 99% homology with P. aeruginosa and could be of novel species and the sequence of the strain was deposited in Genbank with the accession number JX104229.