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Optimization of submerged fermentation of Thelephora ganbajun zang
Author(s) -
Zhang Peng,
Luo XueYue,
Wu Yang,
Chen YiMing,
Xu WeiJian,
Yi XinXin
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200757
Subject(s) - yield (engineering) , fermentation , biomass (ecology) , sucrose , chemistry , selenium , response surface methodology , food science , chromatography , biology , agronomy , materials science , organic chemistry , metallurgy
The effects of the fermentation conditions on both the biomass yield and the organic selenium yield of Thelephora ganbajun zang were studied. The components most suitable for the submerged fermentation medium were examined using the orthogonal array method; they comprised sucrose at 30 g L −1 , carbamide 1 g L −1 , corn steep liquor 8 g L −1 , MgSO 4 ·7H 2 O 0.3 g L −1 , KH 2 PO 4 0.5 g L −1 , and NaCl 5 g L −1 . The optimum cultivation conditions that resulted in maximal biomass yield were obtained using the response surface methodology (RSM). The conditions were as follows: initial pH, 5.84; temperature, 26.16 °C; and rotation speed, 170 rpm. Feeding sucrose led to a higher biomass yield, with a maximum of 21.20 g L −1 . The biomass yield and the organic Se yield of T. ganbajun could reach 10.8 g L −1 and 3256.07 mg kg −1 , respectively, in a culture medium supplemented with 200 mg L −1 sodium selenite (Na 2 SeO 3 ), which was added to the medium at 36 h after inoculation. Application of the orthogonal array method and RSM gave rise to a significant enhancement in the biomass yield of T. ganbajun . The results of these experiments indicate that T. ganbajun is a promising microorganism for selenium enrichment.