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Relationship between membrane damage, leakage of intracellular compounds, and inactivation of Escherichia coli treated by pressurized CO 2
Author(s) -
Yao Chunyan,
Li Xiaodong,
Bi Weiwei,
Jiang Chen
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200640
Subject(s) - intracellular , escherichia coli , leakage (economics) , membrane , chemistry , biophysics , cell membrane , microbiology and biotechnology , biochemistry , biology , gene , macroeconomics , economics
The relationship between membrane damage, leakage of intracellular compounds, and inactivation of Escherichia coli treated by pressurized CO 2 was investigated by assessing the inactivation, bacterial cell membrane permeability, the leakage of protein, nucleic acid, and K + and Mg 2+ of E. coli . The results indicated that pressurized CO 2 treatment induced the leakage of protein in E. coli , but the time of leakage was lagged behind the time of 99% E. coli inactivation, so it was only the secondary phenomenon of inactivation. The inactivation of E. coli was related to the leakage of nucleic acid, K + , Mg 2+ induced by the pressurized CO 2 treatment. There was direct relationship between the inactivation of E. coli and the damaging effect of pressurized CO 2 treatment on the cell membrane of E. coli .