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Cultivable actinomycetes from rhizosphere of birch ( Betula pendula ) growing on a coal mine dump in Silets, Ukraine
Author(s) -
Ostash Bohdan,
Gren Tetiana,
Hrubskyy Yaroslav,
Tistechok Stepan,
Beshley Stepan,
Baranov Volodymyr,
Fedorenko Victor
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200551
Subject(s) - rhizosphere , siderophore , botany , biology , betula pendula , 16s ribosomal rna , streptomyces , bacteria , genetics
Five actinomycete strains were isolated from the rhizosphere of birch, one of a few native tree forms capable of thriving on the upper level of a coal mine dump near the village of Silets (Lvivska region, Ukraine). No such strains were isolated from surrounding gangue, or from nearby grass Calamagrostis epigeios . Using 16S rDNA sequencing and analysis of cell wall aminoacids, four of these strains were shown to belong to genus Streptomyces and one to be Amycolatopsis . The isolates were able to produce siderophores and antibacterial compounds. In comparison to the reference strain Streptomyces coelicolor M145, certain rhizospheric isolates displayed somewhat increased survival in the presence of copper, iron(III), or chromium(VI) salts. The Amycolatopsis isolate was also shown to accumulate significant quantities of heavy metals from waste extracts. Possible roles of the described strains in coal mine dump ecology are discussed.