Premium
An adenosine kinase in apoplastic location is involved in Magnaporthe oryzae cold acclimation
Author(s) -
Li Jian,
Jia Baolei,
Liang Xilong,
Liu Jinliang,
Wang Yanli,
Liang Xunna,
Yan Hai,
Wang Yuhan,
Zhang Shihong
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200481
Subject(s) - apoplast , cytosol , acclimatization , microbiology and biotechnology , biology , biochemistry , cold shock domain , chemistry , botany , enzyme , gene , cell wall , rna
Cold acclimation is an important process to increase freezing tolerance for over‐winter survival in many organisms. The apoplastic area is very important in cold acclimation. Two‐dimensional electrophoresis was used to identify apoplastic proteins involved in the cold acclimation process of the filamentous fungus Magnaporthe oryzae , and nine protein spots showed at least 1.5‐fold increase during cold treatment. These proteins were further analyzed by matrix‐assisted laser‐desorption/ionization time‐of‐flight mass spectrometry. One of these proteins was identified to be an adenosine kinase (MoAK), an ortholog of the adenosine kinase from Saccharomyces cerevisiae . The MoAK gene showed significantly increased in transcription level. Microscopic analyses showed that an MoAK::GFP fusion protein was localized in the apoplastic region. The MoAk protein showed anti‐freezing activity when expressed in yeast. These results indicated that cold acclimation is crucial for fungal freezing tolerance and MoAK played an important role in this process in M. oryzae .