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Efficacy of Ganoderma sp. JAS4 in bioremediation of chlorpyrifos and its hydrolyzing metabolite TCP from agricultural soil
Author(s) -
Silambarasan Sivagnanam,
Abraham Jayanthi
Publication year - 2014
Publication title -
journal of basic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.58
H-Index - 54
eISSN - 1521-4028
pISSN - 0233-111X
DOI - 10.1002/jobm.201200437
Subject(s) - metabolite , chlorpyrifos , bioremediation , biodegradation , degradation (telecommunications) , chemistry , strain (injury) , nutrient , reaction rate constant , food science , environmental chemistry , biology , pesticide , agronomy , biochemistry , kinetics , organic chemistry , contamination , ecology , telecommunications , physics , anatomy , quantum mechanics , computer science
A novel fungal strain JAS4 was isolated from agricultural soil and was found to be highly effective in degrading chlorpyrifos and its major degradation product 3,5,6‐trichloro‐2‐pyridinol (TCP). The molecular characterization based on 18S rRNA sequence analysis, revealed strain JAS4 as Ganoderma sp. which could able to degrade chlorpyrifos and its metabolite in an aqueous medium with rate constant of 0.8460 day −1 , following first order rate kinetics, and the time in which the initial insecticide concentration was reduced by 50% (DT 50 ) was 0.81 days. Studies on biodegradation in soil with nutrients showed that JAS4 strain exhibited efficient degradation of insecticide with a rate constant of 0.9 day −1 , and DT 50 was 0.73 day. In contrast, degradation of insecticide in soil without nutrients was characterized by a rate constant of 0.7576 day −1 and the DT 50 was 0.91 day.